Core Four

With over 40 assays for various organisms and genes, our molecular toolbox has a solution for any spoiler or organism that will grow in your beer, wine, kombucha or general beverage. To give our users the ability to quantify anything that could spoil or is used to intentionally ferment your beverage we have our “Core Four” assays. The Core Four is the perfect starting point to make sure your beverage is spoiler free.

  • CER Assay

    Screens for all general Saccharomyces strains

  • STA+ Assay

    Screens for all S. cerevisiae var. diastaticus genes and the promoter gene for highly diastatic strains

  • BRETT ALL Assay

    Screens for all strains of Brettanomyces (Brettanomyces bruxellensis, Brettanomyces anomala (Brett claussenii), Brettanomyces naardenensis, Brettanomyces nanus, Brettanomyces custersiana) along with other wild yeasts such as Candida, Pichia, Issatchenkia, Ambrosiozyma, and Hanseniaspora.

  • HOR ABC Assay

    Screens for the HorA, HorB, and HorC genes responsible for hop resistance in Lactobacillus, Pediococcus, Oenococcus, and Staphylococcus.

With the Core Four you'll be able to rapidly quantify levels of brewers yeast. If filtering or pasteurizing your sample, you’ll be able to confirm proper pasteurization or filtering by targeting your general saccharomyces. In the event of an infection you'll have immediate answers as to whether or not your sample is highly or poorly diastatic, if any bacteria is present and specifically what level of hop resistance the strain has, and if you have strains of Brettanomyces or wild yeast. 

Since our system is built on proven quantitative polymerase chain reaction (qPCR) technology you will be able to get a specific range from 1 cell per mL all the way up to 5 billion cells per mL without enrichment.

If you do get a positive hit on either the HOR ABC or BRETT ALL assays, you can identify the organism(s) present by simply running the auxiliary strain-level assays. Our sample prep workflows yield enough DNA templates to run all of our assays at once, so there's no need to prep another sample to run additional assays beyond the Core Four. Since the Core Four is so comprehensive most users stick to just these tests. However it's essential to have the other assays when you need them to get immediate and extensive answers on the risk a detected beer, wine or kombucha contamination poses.

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